1 Scope
This standard specifies the determination method of aflatoxin M1 and aflatoxin M2 (hereinafter referred to as AFT M1 and AFT M2) in foods.
Method I is isotope dilution-liquid chromatography-tandem mass spectrometry, it is applicable to the determination of AFT M1 and AFT M2 in milk, milk products and milk-containing foods for special dietary supplies.
Method II is high performance liquid chromatography, its application scope is the same with Method I.
Method III is enzyme linked immunosorbent assay screening method, it is applicable to the screening determination of AFT M1 in milk, milk products and milk-containing foods for special dietary supplies.
Method I Isotope Dilution-Liquid Chromatography-Tandem Mass Spectrometry
2 Principle
After AFT M1 and AFT M2 in the sample extracted by methanol-water solution and dilute the supernatant with water or phosphate buffer solution, purified and enriched by immunoaffinity column, scale the volume and filter, the filtrate can be separated by liquid chromatography, tested by tandem mass spectrometry and quantified by isotope internal standard method.
3 Reagents and Materials
Unless otherwise specified, reagents used in this method are all analytically pure, and water is the Grade 1 water as specified in GB/T 6682.
3.1 Reagents
3.1.1 Acetonitrile (CH3CN): chromatographically pure.
3.1.2 Methanol (CH3OH): chromatographically pure.
3.1.3 Ammonium acetate (CH3COONH4).
3.1.4 Sodium chloride (NaCl).
3.1.5 Disodium hydrogen phosphate (Na2HPO4).
3.1.6 Sodium dihydrogen phosphate (KH2PO4).