1 Scope
This standard specifies the determination methods of aflatoxin B1, aflatoxin B2, aflatoxin G1 and aflatoxin G2 (hereinafter referred to as AFT B1, AFT B2, AFT G1 and AFT G2) in foods.
Method I in this standard is isotope-dilution liquid chromatography-tandem mass spectrometry, which is applicable to the determination of AFT B1, AFT B2, AFT G1 and AFT G2 in cereals and their products, beans and their products, nuts and seeds, oils and their products, condiments, formula foods for infants and young children and complementary foods for infants and young children.
Method II in this standard is high performance liquid chromatography-pre-column derivatization method, which is applicable to the determination of AFT B1, AFT B2, AFT G1 and AFT G2 in cereals and their products, beans and their products, nuts and seeds, oils and their products, condiments, formula foods for infants and young children and complementary foods for infants and young children.
Method III in this standard is high performance liquid chromatography - post-column derivatization method, which is applicable to the determination of AFT B1, AFT B2, AFT G1 and AFT G2 in cereals and their products, beans and their products, nuts and seeds, oils and their products, condiments, formula foods for infants and young children and complementary foods for infants and young children.
Method IV in this standard is enzyme-linked immunosorbent screening method, which is applicable to the determination of AFT B1 in cereals and their products, beans and their products, nuts and seeds, oils and their products, condiments, formula foods for infants and young children and complementary foods for infants and young children.
Method V in this standard is thin layer chromatography, which is applicable to the determination of AFT B1 in cereals and their products, beans and their products, nuts and seeds, oils and their products and condiments.
Method I Isotope-dilution Liquid Chromatography-tandem Mass Spectrometry
2 Principle
Extract the aflatoxin B1, aflatoxin B2, aflatoxin G1 and aflatoxin G2 in the specimen with acetonitrile-water solution or methanol-water solution, dilute the extracting solution with phosphate buffer solution containing 1% TritonX-100 (or Tween-20) (where necessary, preliminary purification by aflatoxin solid-phase purification column shall be carried out), purify and enrich through immunoaffinity column, concentrate the purified solution, scale the volume, separate with liquid chromatograph after filtration, examine with tandem mass spectrum and quantify with isotope internal standard method.
3 Reagents and Materials
Unless otherwise specified, analytically-pure reagents and Class-I water (defined in GB/T 6682) are adopted for the purpose of this method.
3.1 Reagents
3.1.1 Acetonitrile (CH3CN): chromatographically pure.
3.1.2 Methanol (CH3OH): chromatographically pure.
3.1.3 Ammonium acetate (CH3COONH4): chromatographically pure.
3.1.4 Sodium chloride (NaCl).
3.1.5 Disodium hydrogen phosphate: (Na2HPO4).
Foreword i
1 Scope
2 Principle
3 Reagents and Materials
4 Instruments and Apparatus
5 Analysis Steps
6 Expression of Analysis Results
7 Accuracy
8 Other
9 Principle
10 Reagents and Materials
11 Instruments and Apparatus
12 Analysis Steps
13 Expression of Analysis Results
14 Accuracy
15 Other
16 Principle
17 Reagents and Materials
18 Instruments and Apparatus
19 Analysis Steps
20 Expression of Analysis Results
21 Accuracy
22 Other
23 Principle
24 Reagents and Materials
25 Instruments and Apparatus
26 Analysis Steps
27 Expression of Analysis Results
28 Accuracy
29 Other
30 Principle
31 Reagents and Materials
32 Instruments and Apparatus
33 Analysis Steps
34 Accuracy
35 Other
Annex A Calibration Methods for Standard Concentrations of AFT B1, AFT B2, AFT G1 and AFT G
Annex B Verification Methods for Immunoaffinity Column
Annex C Tandem Mass Spectrometry
Annex D Liquid Chromatogram
Annex E Judgment Method for the Quality of Enzyme Linked Immunosorbent Assay Kit
