1 Scope
This standard specifies the methods of high performance liquid chromatography and fluorophotometry for determining Vitamin B1 in foods.
This standard is applicable to the determination of Vitamin B1 in foods.
Method I High Performance Liquid Chromatography
2 Principle
Hydrolyze and neutralize the specimen in the medium of diluted hydrochloric acid, carry out enzymolysis for the specimen, derive the hydrolysate with alkaline potassium ferricyanide solution, extract with n-butyl alcohol, separate with C18 reversed-phase chromatographic column, inspect with high performance liquid chromatography-fluorescence detector, and quantify by using external standard method.
3 Reagents and Materials
Unless otherwise specified, analytically-pure reagents and Grade I water (defined in GB/T 6682) are adopted for the purposes of this method.
3.1 Reagents
3.1.1 n-butyl alcohol (CH3CH2CH2CH2OH)。
3.1.2 Potassium ferricyanide [K3Fe(CN)6]。
3.1.3 Sodium hydroxide (NaOH)。
3.1.4 Hydrochloric acid (HCl)。
Contents
Foreword i
1 Scope
2 Principle
3 Reagents and Materials
4 Instruments and Apparatus
5 Analysis Steps
6 Expression of Analysis Results
7 Precision
8 Others
9 Principle
10 Reagents and Materials
11 Instruments and Apparatus
12 Analysis Steps
13 Expression of Analysis Results
14 Precision
15 Others
Appendix A High Performance Liquid Chromatogram of Vitamin Standard Derivative
