1 Scope
This standard specifies the screening method through reversed-phase high performance liquid chromatography (RHPLC), the determination method through normal phase high performance liquid chromatography (N-HPLC) and liquid chromatography - tandem mass spectrum (LC-MS/MS) all regarding alkylphenol polyoxyethylene (APnEO, n=2~16 ) in textiles.
This standard is applicable to various textile products.
Note: The general formula for molecular structure of APnEO is: R-C6H4-(OC2H4)nOH. The “APnEO” in this standard refers to common octaphenyl polyoxyethylene ether [OPnEO, C8H17-C6H4-(OC2H4)nOH] and nonylphenol polyoxyethylene ether [NPnEO, C9H19-C6H4-(OC2H4)nOH].
2 Principle
Extract APnEO in sample through Soxhlet extraction method with methyl alcohol serving as the extraction solvent, and after having concentrated and purified the extracting solution, determine such extracting solution with high-performance liquid chromatograph equipped with fluorescence detector or through liquid chromatography - tandem mass spectrum, then quantify with external standard method.
Foreword I
1 Scope
2 Principle
3 Reagents and Standard Solutions
4 Apparatus and Materials
5 Analysis Procedures
6 Determination Lower Limits, Recovery Rate and Precision of the Test Method
7 Test Report
Appendix A (Informative) RHPLC Reference Retention Time and Chromatogram for Standard Alkylphenol Ethoxylates
Appendix B (Informative) N-HPLC Reference Retention Time and Chromatogram for Standard Alkylphenol Ethoxylates
Appendix C (Informative) Reference Conditions for Electrospray Ion Source of API 4000 LC-MS/MS System
