1 Scope
This standard specifies the grouping detection of vibrio cholerae in food - MPCR-DHPLC, including the vibrio cholerae of O1 group, O139 group and non O1/non O139 group.
This standard is applicable to the fast grouping detection of vibrio cholerae in food - MPCR-DHPLC, including the vibrio cholerae of O1 group, O139 group and non O1/non O139 group.
2 Normative References
The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the reference document (including all the amendments) applies.
GB/T 6682 Water for Analytical Laboratory Use - Specification and Test Methods
GB 19489 Laboratories - General Requirements for Biosafety
GB/T 27403 Criterion on Quality Control of Laboratories - Molecular Biological Testing of Food
SN/T 1022 Method for the Determination of Vibrio Cholerae in Food for Export
3 Abbreviations
For the purpose of this standard, the following abbreviations apply.
3.1
PCR polymerase chain reaction
Foreword i
1 Scope
2 Normative References
3 Abbreviation
4 Biosafety Measures
5 Measures for Waste Disposal and Anti-pollution
6 Principle
7 Reagents and Materials
8 Main Instruments and Equipment
9 Method Summary and Detection Procedure
10 Operation Steps
11 Result and Judgment
Appendix A (Normative) Primer Sequence Used for Grouping Detection of Vibrio Cholerae in Foods with MPCR-DHPLC Method
