GB/T 46257-2025 Determination of acetylcholine and its salts in cosmetics—Liquid chromatography-tandem mass spectrometry English, Anglais, Englisch, Inglés, えいご
This is a draft translation for reference among interesting stakeholders. The finalized translation (passing thorugh draft translation, self-check, revision and varification) will be delivered upon being ordered.
ICS 71.100.70 CCS Y 42
National Standard of the People's Republic of China
GB/T 46257-2025
Determination of Acetylcholine and Its Salts in Cosmetics
Liquid Chromatography-Tandem Mass Spectrometry
Issued on: 2025-08-29 Implementation: 2026-03-01
Issued by: State Administration for Market Regulation, Standardization Administration of China
Content
Preface
Introduction
1 Scope
2 Normative References
3 Terms and definitions
4 Principles
5 Reagents and Materials
6 Instruments and equipment
7 Sample Preparation and Testing Procedures
8 Experimental data processing
9 Recovery rate
10 Precision
11 Test Report
1 Scope
This document specifies the principle, reagents, instruments, sample preparation, experimental procedures, data processing, recovery rate, precision, and test reporting for the determination of acetylcholine and its salts in cosmetics by liquid chromatography-tandem mass spectrometry (LC-MS/MS).
Applicability:
Cosmetic types: Aqueous solutions, creams, emulsions, and oils.
Method detection limit (MDL): 0.05 mg/kg (acetylcholine equivalent).
Method quantification limit (MQL): 0.1 mg/kg.
2 Normative References
GB/T 6682 Requirements for water in analytical laboratories (latest version).
3 Terms and Definitions
No terms require definition.
4 Principle
Sample extraction, Vortex oscillation with 50% acetonitrile solution. Ultrasonic extraction. Lipid removal using n-hexane. Centrifugation and filtration.
