GB 7300.206-2025 Feed additives—Part 2: Vitamins,provitamins and chemically well-defined substances having similar effects—Choline chloride English, Anglais, Englisch, Inglés, えいご
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ICS
CCS H
National Standard of the People's Republic of China
GB 7300.206-2025
Replaces GB 34462-2017
Feed additives - Part 2: Vitamins, provitamins and chemically well-defined substances having similar effects - Choline chloride
饲料添加剂 第2部分：维生素及类维生素 氯化胆碱
Issue date: 2025-12-31 Implementation date: 2027-01-01
Issued by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
the Standardization Administration of the People's Republic of China
Contents
Foreword
Introduction
1 Scope
2 Normative References
3 Terms and Definitions
4 Chemical Name, Molecular Formula, Relative Molecular Mass and Structural Formula
5 Raw Materials
6 Technical Requirements
7 Sampling
8 Test Methods
9 Inspection Rules
10 Labeling, Packaging, Transportation, Storage and Shelf Life
Introduction
Feed additives refer to substances added in small or trace amounts during the processing, manufacturing, and use of feed, including nutritional feed additives and general feed additives. For ease of use, and according to product type, GB 7300 "Feed Additives" is divided into the following 13 parts:
— Part 1: Amino acids, amino acid salts, and analogues;
— Part 2: Vitamins and vitamin-like substances;
— Part 3: Mineral elements and their complexes (chelates);
— Part 4: Enzyme preparations;
— Part 5: Microorganisms;
— Part 6: Non-protein nitrogen;
— Part 7: Antioxidants;
— Part 8: Preservatives, mold inhibitors, and acidity regulators;
— Part 9: Coloring agents;
— Part 10: Flavoring and appetite-stimulating substances;
— Part 11: Binders, anti-caking agents, stabilizers, and emulsifiers;
— Part 12: Polysaccharides and oligosaccharides;
— Part 13: Others.
The product choline chloride covered by this document belongs to Part 2: Vitamins and vitamin-like substances. As choline chloride is the sixth product standard published under this major category, this document is assigned the number GB 7300.206 and serves as Part 206 of GB 7300.
Feed Additives - Part 2: Vitamins and Vitamin-Like Substances - Choline Chloride
1 Scope
This document provides the chemical name, molecular formula, relative molecular mass and structural formula of choline chloride, and specifies the raw materials, technical requirements, inspection rules, labeling, packaging, transportation, storage, and shelf life of the feed additive choline chloride. It also describes the sampling and test methods.
This document applies to the feed additive choline chloride aqueous solution produced by the reaction of trimethylamine hydrochloride aqueous solution with ethylene oxide, and the feed additive choline chloride powder produced by adding a carrier to the choline chloride aqueous solution as raw material.
2 Normative References
The contents of the following documents constitute essential provisions of this document through normative reference. For dated references, only the edition corresponding to the date applies. For undated references, the latest edition (including any amendments) applies.
GB/T 601 Chemical Reagents - Preparations of Standard Volumetric Solutions
GB/T 6682 Water for Analytical Laboratory Use - Specification and Test Methods
GB/T 8170 Rules of Rounding Off for Numerical Values & Expression and Judgment of Limiting Values
GB/T 9724 Chemical Reagents - General Rules for the Determination of pH
GB 10648 Feed Labeling
GB 13078 Hygienic Standard for Feeds
GB/T 13079 Determination of Total Arsenic in Feeds
GB/T 14699 Feeding Stuffs - Sampling
NY/T 4689 Test for Limit of Heavy Metals in Feed Additives - Turbidimetric Method
3 Terms and Definitions
No terms and definitions are stipulated in this document.
4 Chemical Name, Molecular Formula, Relative Molecular Mass and Structural Formula
4.1 Chemical Name
2-Hydroxyethyltrimethylammonium chloride.
4.2 Molecular Formula
C₅H₁₄ClNO.
4.3 Relative Molecular Mass
139.65 (based on the 2022 international relative atomic masses).
4.4 Structural Formula
Shown in Figure 1.
5 Raw Materials
The carrier used in the feed additive choline chloride powder shall be sourced from the Feed Ingredients Catalog and the Feed Additive Varieties Catalog. Feed ingredients shall comply with the requirements of GB 13078.
6 Technical Requirements
6.1 Appearance and Properties
6.1.1 Aqueous Solution
Shall be a colorless, transparent viscous liquid with a specific odor.
6.1.2 Powder
Shall be a white or yellowish-brown dry, free-flowing powder or granular material, hygroscopic, with a specific odor.
6.2 Identification
Shall meet the requirements of the identification test for the aqueous solution and powder samples.
6.3 Physicochemical Indicators
Shall comply with the requirements specified in Table 1.
6.4 Hygiene Indicators
Shall comply with the requirements specified in Table 2.
7 Sampling
Carry out according to GB/T 14699.
8 Test Methods
Warning - Some reagents used in the test methods are corrosive and toxic. Exercise caution during operation and take appropriate safety and protective measures.
8.1 General Provisions
Unless otherwise specified, all reagents used shall be of analytical grade; all standard volumetric solutions shall be prepared and standardized according to GB/T 601; water for chromatographic tests shall be Grade 1 water as specified in GB/T 6682, and water for other tests shall be Grade 3 water as specified in GB/T 6682.
8.2 Appearance and Properties
8.2.1 Aqueous Solution
Place an appropriate amount of the aqueous solution sample in a clean, dry beaker. Observe its color and state under natural light, and smell its odor.
8.2.2 Powder
Take an appropriate amount of the powder sample and place it on a clean, dry white porcelain plate. Observe its color and state under natural light, and smell its odor.
8.3 Identification
8.3.1 Reagents or Materials
8.3.1.1 Diammonium tetrathiocyanatodiamminechromate (Reinecke's salt).
8.3.1.2 Potassium hydroxide.
8.3.1.3 Potassium permanganate.
8.3.1.4 Nitric acid solution: Transfer 10 ml of nitric acid, dilute with water to 100 ml, and mix well.
8.3.1.5 Sulfuric acid solution: Transfer 6 ml of sulfuric acid, slowly add it to approximately 94 ml of water while stirring (avoid excessive heat generation), cool, and mix well.
8.3.1.6 Ammonia solution: Transfer 10 ml of ammonia water, dilute with water to 100 ml, and mix well.
8.3.1.7 Reinecke's salt methanol solution: Weigh 2 g of Reinecke's salt (8.3.1.1), dissolve in 100 ml of methanol, and filter. The validity period is 48 hours.
8.3.1.8 Potassium tetraiodomercurate(II) solution: Weigh 1.36 g of mercury(II) chloride, dissolve in 60 ml of water. Separately, weigh 5 g of potassium iodide, dissolve in 10 ml of water. Mix the two solutions, dilute with water to 100 ml, and mix well.
8.3.1.9 Silver nitrate solution: Weigh 1.7 g of silver nitrate, dissolve in water and dilute to 100 ml, mix well.
8.3.1.10 Red litmus paper.
8.3.1.11 Starch-potassium iodide test paper.
8.3.2 Apparatus
Analytical balance: accuracy 0.01 g.
