标准编号:	GB 22255-2014
中文名称:	食品安全国家标准 食品中三氯蔗糖（蔗糖素）的测定
英文名称:	National Food Safety Standard Determination of Sucralose in Foods
中标分类:	X09    卫生、安全、劳动保护
行业分类:	GB    国家标准
发布机构:	国家卫生和计划生育委员会
发布日期:	2015-01-28
实施日期:	2015-7-28
标准状态:	被替代
被新标准替代:	GB 5009.298-2023 食品安全国家标准 食品中三氯蔗糖(蔗糖素)的测定
被替代日期:	2024-9-6
替代旧标准:	GB/T 22255-2008 食品中三氯蔗糖（蔗糖素）的测定
翻译语言:	英文
文件格式:	PDF
中文字符数:	6000 字
翻译价格(元):	200.00 元
交付时间:	付款后 1 个工作日

1 Scope



This standard specifies the determination method of sucralose in foods.



This standard is applicable to the determination of sucralose in foods.



2 Theory



Extract the sucralose in the specimen with methanol aqueous solution, remove protein and fat, then separate it with high performance liquid chromatograph and reversed-phase C18 chromatographic column after purifying and gathering it with solid-phase extraction column, detect it with evaporative light-scattering detector or refractive index detector, conduct qualitative analysis according to retention time, and quantify it by peak area.



3 Reagents and Materials



Note: Unless otherwise specified, analytically pure reagents and Grade 1 water (specified in GB/T 6682) are adopted for the purpose of this method.



3.1 Reagents



3.1.1 Methanol (CH3OH).



3.1.2 Acetonitrile (CH3CN): chromatographically pure.



3.1.3 N-hexane (C6H14).



3.1.4 Zinc acetate [Zn(CH3COO)2•2H2O].

Foreword i
1 Scope
2 Theory
3 Reagents and Materials
4 Apparatuses
5 Analytical Procedures
6 Expression of Analysis Results
7 Precision
8 Others
Annex A The Elution Program of Liquid Chromatography under Complex Conditions (Applicable to Evaporative Light-scattering Detector)
Annex B The High performance Liquid Chromatograms of Sucralose