1 Scope
This standard specifies the high performance liquid chromatography determination method of thiamine hydrochloride, pyridoxine hydrochloride, niacin, niacinamide and caffeine in health foods.
This standard is applicable to the high performance liquid chromatography determination method for adding thiamine hydrochloride, pyridoxine hydrochloride, niacin, niacinamide and caffeine serving as dietary supplements into test sample types such as tablet, capsule, oral liquid and drink.
Detection limit of this method is: 0.02μg for thiamine hydrochloride, 0.02μg for pyridoxine hydrochloride, 0.10μg for niacin, 0.06μg for niacinamide and 0.05μg for caffeine. The linear range is: 0.500μg/mL~50.0μg/mL for thiamine hydrochloride; 0.500μg/mL~50.0μg/mL for pyridoxine hydrochloride; 1.00μg/mL~100μg/mL for niacin; 1.00μg/mL~100μg/mL for niacinamide; and 5.00μg/mL~500μg/mL for caffeine.
