Foreword
Codeofchina.com is in charge of this English translation. In case of any doubt about the English translation, the Chinese original shall be considered authoritative.
This standard replaces GB 5009.8-2016 National food safety standard - Determination of fructose, glucose, sucrose, maltose and lactose in foods and GB 5413.5-2010 National food safety standard - Determination of lactose and sucrose in foods for infants and young children, milk and milk products.
The following main changes have been made with respect to GB 5009.8-2016:
——The application scope of Method I - high-performance liquid chromatography has been modified;
——The ion chromatography has been added as Method II;
——The acid hydrolysis-Lane-Eynon method has been modified as Method III;
——The Lane-Eynon method has been added as Method IV.
National food safety standard -
Determination of fructose, glucose, sucrose, maltose and lactose in foods
1 Scope
This standard specifies the methods for determination of fructose, glucose, sucrose, maltose and lactose in foods.
Method I - High-performance liquid chromatography, applicable to the determination of fructose, glucose, sucrose, maltose and lactose in grain and grain products, milk and milk products, fruits and vegetables and fruit and vegetable products, sweeteners, candies and beverages, as well as fructose, glucose, sucrose, maltose and lactose in foods for infant and young children.
Method II - Ion chromatography, applicable to the determination of fructose, glucose, sucrose, maltose and lactose in foods.
Method III - Acid hydrolysis-Lane-Eynon method, applicable to the determination of sucrose in foods.
Method IV - Lane-Eynon method, applicable to the determination of lactose in foods for infants and young children and lactose in milk products.
Method I High-performance liquid chromatography
2 Principle
Fructose, glucose, sucrose, maltose and lactose in the specimens are extracted, and separated by high-performance liquid chromatography (HPLC) columns, detected by a differential refraction detector or an evaporative light scattering detector, and quantified by external standard method.
3 Reagents and materials
Unless otherwise stated, reagents used in this method are all analytically pure, and water is of Grade 1 as specified in GB/T 6682.
3.1 Reagents
3.1.1 Acetonitrile (C2H3N): Chromatographically pure.
3.1.2 Zinc acetate [Zn(CH3COO)2•2H2O].
3.1.3 Potassium ferrocyanide [K4Fe(CN)6•3H2O].
3.1.4 Glacial acetic acid (CH3COOH).
3.2 Reagent preparation
3.2.1 Zinc acetate solution (1mol/L): Weigh out 21.9g of zinc acetate and add 3mL of glacial acetic acid; dissolve the mixture in water, dilute the solution to 100mL and mix it well.
3.2.2 Potassium ferricyanide solution (0.25mol/L): Weigh out 10.6g of potassium ferricyanide, dissolve it in water, dilute the solution to 100 mL and mix it well.
3.3 Standard substance
3.3.1 Fructose (C6H12O6, CAS No. 57-48-7): With a purity ≥99%, or a standard substance certified by the state and granted a standard substance certificate.
3.3.2 Glucose (C6H12O6, CAS No. 50-99-7): With a purity ≥99%, or a standard substance certified by the state and granted a standard substance certificate.
3.3.3 Sucrose (C12H22O11, CAS No. 57-50-1): With a purity ≥99%, or a standard substance certified by the state and granted a standard substance certificate.
3.3.4 Maltose (C12H22O11, CAS No. 69-79-4): With a purity ≥99%, or a standard substance certified by the state and granted a standard substance certificate.
3.3.5 Lactose (C12H22O11, CAS No. 63-42-3): With a purity ≥99%, or a standard substance certified by the state and granted a standard substance certificate.
3.4 Preparation of standard solutions
Foreword II
1 Scope
2 Principle
3 Reagents and materials
4 Apparatuses
5 Analytical procedures
6 Expression of analysis results
7 Precision
8 Others
9 Principle
10 Reagents and materials
11 Apparatuses
12 Test procedures
13 Expression of analysis results
14 Precision
15 Others
16 Principle
17 Reagents and solutions
18 Apparatuses
19 Analytical procedures
20 Expression of analysis results
21 Precision
22 Principle
23 Reagents and materials
24 Apparatuses
25 Analytical procedures
26 Expression of analysis results
27 Precision
Annex A High-performance liquid chromatograms of standard solutions of fructose, glucose, sucrose, maltose and lactose
Annex B Ion chromatogram of standard solutions of fructose, glucose, sucrose, maltose and lactose
