NY/T 2890-2016 Determination of γ-aminobutyric acid in rice - HPLC
1 Scope
This standard specifies the high performance liquid chromatography for determination of γ-aminobutyric acid content in rice.
This standard is applicable to the determination of γ-aminobutyric acid content in rice.
For the purpose of this method, the limit of detection is 0.10 mg/kg, and the limit of quantification is 0.20 mg/kg.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
GB/T 6682 Water for analytical laboratory use - Specification and test methods
3 Principle
The specimen is extracted with ethanol aqueous solution, derived with 4-dimethylaminoazobenzene-4-sulfonyl chloride (DABS-Cl), and determined by high performance liquid chromatography, with retention time for qualitative analysis and external standard method for quantitative analysis.
4 Reagents and materials
Unless otherwise specified, analytical grade reagents shall be used. Class-I water specified in GB/T 6682 is adopted.
4.1 Anhydrous ethanol (C2H5OH): Ultrapure.
4.2 Acetonitrile (CH3CN): Chromatographically pure.
4.3 Extraction solution: Anhydrous ethanol + water = 4 + 1 (V + V), mix 400 mL of anhydrous ethanol and 100 mL of water well.
Foreword i
1 Scope
2 Normative references
3 Principle
4 Reagents and materials
5 Instruments and apparatus
6 Analytical procedure
7 Result calculation
8 Precision
Annex A (Informative) Chromatogram of γ-aminobutyric acid standard solution
