1 Scope
This standard specifies the methods for determining 20 types of allergenic disperse dyestuffs in dyed leathers, furs and their products.
This standard is applicable to dyed leather and fur products.
2 Normative References
The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
GB/T 6682 Water for Analytical Laboratory Use - Specification and Test Methods
QB/T 1267 Finished Fur Products - Sample Piece - Position and Marking
QB/T 1272 Finished Fur Products - Preparation of Chemical Analysis Sample and General Rules for Chemical Analysis
QB/T 2706 Leather - Chemical Physical and Mechanical and Fastness Tests - Sampling Location
QB/T 2716 Leather - Preparation of Chemical Test Samples
3 Theory
After the sample was extracted with methanol under ultrasonic wave, subject the extract liquor to qualitative and quantitative determination with the high performance liquid chromatography - diode array detector (HPLC/DAD) or the high performance liquid chromatography - tandem mass spectrometry (HPLC-MS/MS).
4 Reagents
Unless otherwise specified, the analytically pure reagents and Grade II water (specified in GB/T 6682) are adopted.
4.1 Methanol (chromatographic grade).
4.2 Disodium hydrogen phosphate.
Foreword i
1 Scope
2 Normative References
3 Theory
4 Reagents
5 Instruments and Apparatus
6 Analytical Procedures
7 Result Calculation
8 Determination Lower Limit and Precision of the Method
9 Test Report
Annex A (Informative) Retention Time and Maximum Detection Wavelength of Allergenous Disperse Dyestuffs Analyzed by HPLC/DAD
Annex B (Informative) Liquid Chromatograms of Allergenous Disperse Dyestuff Standard Sample (with a Concentration of 5 mg/kg) at Different Detection Wavelengths
Annex C (Informative) UV-visible Absorption Spectrograms of 20 Types of Allergenous Disperse Dyestuff Standard Sample
Annex D (Informative) Mass Spectrometry Parameters of 20 Types of Allergenous Dyestuff Standard Sample
Annex E (Informative) Multi-reactions Monitoring (MRM) Chromatograms for 20 Types of Allergenous Dyestuffs
