GB/T 29578-2013 Detection and identification of Xanthomonas albilineans (Ashby) Dowson
1 Scope
This standard specifies the detection and identification of Xanthomonas albilineans (Ashby) Dowson.
This standard is applicable to the detection and identification of sugarcane leaf scald disease in sugarcane seedlings, stalks and other plant materials.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
GB/T 4789.28 Microbiolgical examination of food hygiene - Staining methods,culture mediums and reagents
SN/T 1193 General requirements for the laboratories for gene detection and identification
SN/T 2122 Sampling for the quarantine of plants and their products for import and export
3 Basic information of Xanthomonas albilineans (Ashby) Dowson
Scientific name: Xanthomonas albilineans (Ashby) Dowson.
English name of the disease: sugarcane leaf scald disease.
Kingdom of Bacteria, phylum of Proteobacteria, class of Gammaproteobacteria, order of Xanthomonodales, family of Xanthomonodaceae and genus of Xanthomonas.
The disease transmits mainly rely on susceptible strains and in a mechanical way; infected sugarcane seeding is the main mode of transmission of the disease, and cutting knives (including loaded mechanized cutters) are also an important source of transmission. Rodents, and pests such as beetles, locusts, leafhoppers, and sugarcane borers are also responsible for the spread of sugarcane leaf scald disease.
See Annex A for other basic information on Xanthomonas albilineans (Ashby) Dowson.
Foreword i
1 Scope
2 Normative references
3 Basic information of Xanthomonas albilineans (Ashby) Dowson
4 Principle
5 Apparatus
6 Reagents and culture medium
7 Methods and procedures for quarantine and identification
8 Result judgment
9 Sample preservation
10 Bacteria preservation
Annex A (Informative) Additional information on Xanthomonas albilineans (Ashby) Dowson
Annex B (Normative) Culture medium preparation and detection method with PCR
