1 Scope
This standard specifies the HPLC-MS-MS determination method of α-trenbolone, β-trenbolone, nortestosterone and epi-nortestosterone residues in milk and milk powder.
This standard is applicable to the determination and confirmation of α-trenbolone, β-trenbolone, nortestosterone and epi-nortestosterone residues in milk and milk powder.
The method detection limits of this standard: for α-trenbolone, β-trenbolone, nortestosterone and epi-nortestosterone in milk, the method detection limit is 1μg/kg; for α-trenbolone, β-trenbolone, nortestosterone and epi-nortestosterone in milk powder, the method detection limit is 5μg/kg.
2 Normative References
The following standards contain provisions which, through reference in this standard, constitute provisions of this standard. For dated reference, subsequent amendments to (excluding corrigendum), or revisions of, any of these publications do not apply. However, parties to agreements based on this standard are encouraged to investigate the possibility of applying the most recent editions of the standards indicated below. For any undated references, the latest edition of the document referred to applies.
GB/T 6379.1 Accuracy (Trueness and Precision) of Measurement Methods and Results - Part 1: General Principles and Definitions (GB/T 6379.1-2004, ISO 5725-1:1994, IDT)
GB/T 6379.2 Accuracy (Trueness and Precision) of Measurement Methods and Results - Part 2: Basic Method for the Determination of Repeatability and Reproducibility of a Standard Measurement Method (GB/T 6379.2-2004, ISO 5725-2:1994, IDT)
GB/T 6682 Water for Analytical Laboratory Use - Specification and Test Methods (GB/T 6682-2008, ISO 3696:1987, MOD)
3 Principle
Foreword i
1 Scope
2 Normative References
3 Principle
4 Reagents and Materials
5 Apparatus
6 Preparation and Preservation of Specimen
7 Determination Procedure
8 Calculation of Result
9 Precision
Appendix A (Informative) Multi-response Monitoring (MRM) Chromatogram of Standards
Appendix B (Informative) Recovery
