Determination of amoxicillin ampicillin piperacillin penicillin G penicillin V oxacillin cloxacillin nafcillin dicloxacillin residues in milk and milk powder - LC-MS-MS method
1 Scope
This standard specifies the LC-MS-MS method for the determination of amoxicillin, ampicillin, piperacillin, penicillin G, penicillin V, oxacillin, cloxacillin, nafcillin, and dicloxacillin residues in milk and milk powder.
This standard is applicable to the determination and confirmation of amoxicillin, ampicillin, piperacillin, penicillin G, penicillin V, oxacillin, cloxacillin, nafcillin, and dicloxacillin residues in milk and milk powder.
The method detection limits specified in this standard are as follows: in milk, 1μg/kg for ampicillin and nafcillin, 2μg/kg for amoxicillin, piperacillin, penicillin G, penicillin V and cloxacillin, and 4μg/kg for oxacillin and dicloxacillin; in milk powder, 8μg/kg for ampicillin and nafcillin, 16μg/kg for amoxicillin, piperacillin, penicillin G, penicillin V and cloxacillin, and 32μg/kg for oxacillin and dicloxacillin.
2 Normative reference
The following normative documents contain provisions which, through reference in this text, constitute provisions of this standard. For dated references, subsequent amendments to (excluding any corrigendum), or revisions of, any of these publications do not apply. However, parties to agreements based on this standard are encouraged to investigate the possibility of applying the latest editions of the standards indicated below. For undated references, the latest edition of the document referred to applies.
GB/T 6379.1 Accuracy (trueness and precision) of measurement methods and results - Part 1: General principles and definitions (GB/T 6379.1-2004, ISO 5725-1:1994, IDT)
GB/T 6379.2 Accuracy (trueness and precision) of measurement methods and results - Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method (GB/T 6379.2-2004, ISO 5725-2:1994, IDT)
GB/T 6682 Water for analytical laboratory use - Specification and test methods (GB/T 6682-2008, ISO 3696:1987, MOD)
3 Principle
The analyte in the specimen is extracted with acetonitrile-water solution, purified by solid phase extraction column, determined by high-performance liquid chromatography-tandem mass spectrometry, and quantified by external standard method.
Foreword i
1 Scope
2 Normative reference
3 Principle
4 Reagents and materials
5 Apparatus
6 Preparation and preservation of specimen
7 Determination procedures
8 Result calculation
9 Precision
Annex A (Informative) Multi reaction monitoring (MRM) chromatograms of reference materials
Annex B (Informative) Recovery rate
