1 Scope
This standard specifies the LC-MS-MS method for the determination of cefazolin, cephapirin, cephalexin, cefalonium, cefquinome residues in honey.
This standard is applicable to the determination of cefazolin, cephapirin, cephalexin, cefalonium, cefquinome residues in honey.
The method detection limit of this standard: cefazolin is 10 μg/kg; cephapirin, cephalexin, cefalonium, cefquinome are 2.0 μg/kg.
2 Normative reference
The following normative documents contain provisions which, through reference in this text, constitute provisions of this standard. For dated references, subsequent amendments to (excluding any corrigendum), or revisions of, any of these publications do not apply. However, parties to agreements based on this standard are encouraged to investigate the possibility of applying the latest editions of the standards indicated below. For undated references, the latest edition of the document referred to applies.
GB/T 6379.1 Accuracy (trueness and precision) of measurement methods and results - Part 1: General principles and definitions (GB/T 6379.1-2004, ISO 5725-1:1994, IDT)
GB/T 6379.2 Accuracy (trueness and precision) of measurement methods and results - Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method (GB/T 6379.2-2004, ISO 5725-2:1994, IDT)
GB/T 6682 Water for analytical laboratory use - Specification and test methods (GB/T 6682-2008, ISO 3696:1987, MOD)
3 Principle
The residues of the five cephalosporins in the specimen are first extracted with sodium dihydrogen phosphate buffer solution and purified with a solid phase extraction column, and then determined by liquid chromatograph mass spectrometer, and quantified by external standard method.
Foreword i
1 Scope
2 Normative reference
3 Principle
4 Reagents and materials
5 Apparatuses
6 Preparation and preservation of specimen
7 Determination procedures
8 Result calculation
9 Precision
Annex A (Informative) Multiple reaction monitoring (MRM) chromatograms of the five cephalosporin reference materials
Annex B (Informative) Recovery rate
