This standard specifies three determination methods for melamine in raw milk, dairy products and products containing milk, i.e. high-performance liquid chromatography (HPLC), liquid chromatography-mass spectrum/mass spectrography (LC-MS/MS) and gas chromatography-mass spectrum [including gas chromatography-mass spectrography (GC-MS), gas chromatography-mass spectrum/mass spectrography (GC-MS/MS)].
This standard is applicable to the quantitative determination of melamine in raw milk, dairy products and products containing milk; liquid chromatography-mass spectrum/mass spectrometry and gas chromatography-mass spectrum/mass spectrography (including gas chromatography-mass spectrum/mass spectrum) are simultaneously applicable to the qualitative corroboration of melamine in raw milk, dairy products and products containing milk.
The quantitation limit of high-performance liquid chromatography in this standard is 2 mg/kg; the quantitation limit of liquid chromatography-mass spectrum/mass spectrometry is 0.01 mg/kg; the quantitation limit of gas chromatography-mass spectrography is 0.05 mg/kg (among which the quantitation limit of gas chromatography-mass spectrum/mass spectrography is 0.005 mg/kg).
Foreword
1 Scope
2 Normative References
3 Method I High-Performance Liquid Chromatography (HPLC)
3.1 Principle
3.2 Reagents and Materials
3.3 Instruments and Equipments
3.5 High-Performance Liquid Chromatography Determination
3.6 Blank Test
3.8 Recovery Rate
3.9 Permissible Deviation
4 Method II Liquid Chromatography-Mass Spectrum/Mass Spectrometry(LC-MS/MS)
4.1 Principle
4.2 Reagents and Materials
4.5 Liquid Chromatography-Mass Spectrum/Mass Spectrum Determination
4.6 Blank Test
4.7 Method Quantitation Limit
4.8 Recovery Rate
4.9 Permissible Deviation
5 Method III Gas Chromatography-Mass Spectrum(GC-MS and GC-MS/MS)
5.1 Principle
5.2 Reagents and Materials
5.3 Instruments and Equipments
5.4 Sample Treatment
5.5 Gas Chromatography-Mass Spectrum Determination
5.6 Blank Test
5.7 Method Quantitation Limit
5.8 Recovery Rate
5.9 Permissible Deviation
Appendix A(Informative)Chromatogram of Standard Melamine
