1 Scope
This standard specifies the enumeration methods of moulds and yeasts in food.
This standard is applicable to the enumeration of moulds and yeasts in various food.
2 Equipments and Materials
Except conventional sterilization in microbiology Lab. and culture equipments, other equipments and materials are listed as follows:
2.1 Refrigerator: 2℃~ 5℃.
2.2 Thermostatic incubator: 28℃±1℃.
2.3 Homogenizer.
2.4 Thermostatic oscillator.
2.5 Microscope: 10×~100×.
2.6 Electronic balance: sensibility 0.1g.
2.7 Sterile conical flask: capacity 500mL, 250mL.
2.8 Sterile jar: 500mL.
2.9 Sterile sucker: 1mL (with 0.01mL scales), 10mL (with 0.1mL scales).
2.10 Sterile plate: diameter 90 mm.
2.11 Sterile test tube: 10mm×75mm.
2.12 Sterile kraft bag and plastic bag.
3 Culture Medium and Reagent
3.1 Potato dextrose agar medium: see A.1 in Annex A.
3.2 Rose Bengal medium: see A.2 in Annex A.
Contents
Foreword
1 Scope
2 Equipments and Materials
3 Culture Medium and Reagent
4 Examination Procedures
5 Operation Steps
6 Results and Reports
Annex A (Normative) Culture Medium and Reagent
Annex B (informative) Direct Microscopy Enumeration Method of Moulds
