1 Scope
This standard specifies the method for the determination of retinol and tocopherol in foods.
This standard is applicable to the determination of retinol and tocopherol in foods.
The detection limits of this standard are: VA: 0.8ng; α-E: 91.8ng; γ-E: 36.6ng; δ-E: 20.6ng.
Method I High Performance Liquid Chromatography
2 Principle
After saponification treatment, retinol and tocopherol in the specimen shall be extracted from the non-saponifiable part into the organic solvent, separated with high performance liquid chromatography C18 reversed phase column, detected by UV detector and then determined quantitatively with internal standard method.
Contents
Foreword I
1 Scope
Method I High Performance Liquid Chromatography
2 Principle
3 Reagents
4 Instruments and Equipment
5 Analysis Procedures
6 Result Calculation
7 Precision
Method 2: Colorimetric Method
8 Principle
9 Reagents
10 Instruments
11 Analysis Procedures
12 Determination
13 Result calculation
14 Precision
