1 Scope
This Standard specifies the determination methods of aflatoxins B1, B2, G1 and G2 in various foods.
Method I and Method II in this Standard are applicable to the determination of aflatoxins B1, B2, G1 and G2 in various foods; Method III in this Standard is applicable to the determination of aflatoxins B1, B2, G1 and G3 in such foods as rice, corn, peanut, almond, walnut and pine nut.
For the purpose of Method I and Method II in this Standard, the minimum detectable quantities are as follows: 0.004μgfor aflatoxins B1 and G1 and 0.002μg for B2 and G2; the minimum detectable concentration are as follows: 5μg/kg for aflatoxins B1 and G1 and 2.5μg/kg for B2 and G2.
For the purpose of Method III, the detection limits of aflatoxins are as follows: 0.50μg/L for B1 and G1 and 0.125μg/L for B2 and G2, which are equivalent to the following concentrations in samples: 0.20μg/kg for B2 and G1 and 0.05μg/kg for B2 and G2. Linear ranges of aflatoxins are as follows: 0.50μg/L~100.0μg/L for B1 and G1 and 0.125μg/L~25.0μg/L for B2 and G2.
2 Normative References
The following standards contain provisions which, through reference in this Standard, constitute provisions of this Standard. For dated reference, subsequent amendments to (excluding corrigendum) or revisions of, any of these publications do not apply. However, all parties coming to an agreement according to this Standard are encouraged to study whether the latest editions of these documents apply. For undated reference, the latest edition of the normative documents referred to applies.
GB/T 5009.22-2003 Method for Determination of Aflatoxin B1 in Foods
Method I Thin Layer Chromatography
3 Principle
After extraction, concentration and thin-layer separation of the specimen, under 365nm ultraviolet light, the aflatoxins B1 and B2 will generate bluish violet fluorescence, while... ...
