GB/T 44836-2024 Textiles - Determination of glyphosate and its salts
WARNING: Persons using this document shall be familiar with normal laboratory practice. This document does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user to establish appropriate safety and health practices and to ensure compliance with any national regulatory conditions.
1 Scope
This document describes the liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of the content of glyphosate and its salts in textiles.
This document is applicable to all kinds of textiles.
2 Normative references
The following documents contain requirements which, through reference in this text, constitute provisions of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
GB/T 6682 Water for analytical laboratory use - Specification and test methods
GB/T 10629 Textiles - Preparation of laboratory test samples and test specimens for chemical testing
3 Terms and definitions
No terms and definitions are listed in this document.
4 Principle
The glyphosate and its salts in the specimen are ultrasonically extracted with water, derivatized with fluorenylmethyl chloroformate (FMOC-CI), and then determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and quantified by internal standard method. Finally, the contents of glyphosate and (aminomethyl) phosphonic acid (the main metabolite) are obtained.
Note: Glyphosate and its salts mainly include glyphosate (CAS No.: 1071-83-6), isopropylammonium salt (CAS No.: 38641-94-0), potassium salt (CAS No.: 70901-12-1), ammonium salt (CAS No.: 40465-66-5), etc. The glyphosate and its salts are extremely soluble in water, their functional substances are glyphosate, and their main metabolites are (aminomethyl) phosphonic acid (AMPA, CAS No.: 1066-51-9). The content of glyphosate and its salts is actually to determine the glyphosate and AMPA (the main metabolite).
5 Reagents
Contents Foreword i 1 Scope 2 Normative references 3 Terms and definitions 4 Principle 5 Reagents 6 Apparatus 7 Test procedure 8 Calculation and expression of results 9 Limit of quantification and precision 10 Test report Annex A (Informative) Example of LC-MS/MS test parameters for glyphosate and its salts Annex B (Informative) LC-MS/MS total ion current chromatograms and multiple reactive ion monitoring (MRM) chromatograms of glyphosate and its salts
