Leather - Determination of antimicrobial activity - Part 1: Film contact method
Caution - The microorganisms involved in this document can be infected and pathogenic. Operators shall take all necessary safeguard measures to avoid harm to personnel and the environment. Tests shall be carried out by professionals trained in microbiology and with practical experience in experimental chamber with technical conditions for handling microorganisms.
1 Scope
This document describes the film contact method, a quantitative test method for the determination of antimicrobial activity of leather.
This document is applicable to the determination of antimicrobial activity of non-absorbent leather.
2 Normative references
The following documents contain requirements which, through reference in this text, constitute provisions of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
QB/T 2707 Leather - Physical and mechanical tests - Sample preparation and conditioning
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
antimicrobial activity
ability to use chemical or physical methods to kill or hinder the growth and reproduction of bacteria and fungi, which can reduce their quantity and activity
[Source: WS/T 650-2019, 3.1, modified]
3.2
control sample
sample that is not antimicrobial treated and is used to verify the growth conditions of the test microorganisms
4 Principle
By inoculating quantitative microorganisms on the surface of non-absorbent leather sample, the microorganisms are uniformly contacted with the surface of the sample by sticking a film. After a certain period of culture, the number of viable bacteria on the surface of the sample is determined, and the antibacterial rate is calculated to express the antibacterial activity of the sample.
Contents
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Instruments and apparatus
6 Reagents and materials
7 Culture medium
8 Test bacteria species
9 Preparation of test bacteria solution
10 Specimen preparation
11 Test procedures
12 Result calculation and expression
13 Test report
Bibliography
