1 Scope
This standard specifies the method for inspection of bacillus subtilis in feeds.
This standard is applicable to the determination and counting of bacillus subtilis in feeds.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
GB/T 8170 Rules of rounding off for numerical values & expression and judgement of limiting values
GB/T 14699.1 Feeding stuffs - Sampling (GB/T 14699.1-2005, IS0 6497:2002, IDT)
GB/T 20195 Animal feeding stuffs - Preparation of test samples (GB/T 20195-2006, IS0 6498: 1998, IDT)
3 Diluent, medium and reagent
Unless otherwise specified, only the recognized analytical reagents shall be used during the analysis, and distilled water or deionized water or water with equivalent purity shall be used as laboratory water.
3.1 0.85% sterilized normal saline.
3.2 Nutrient agar (NA) medium: see A.1 of Annex A.
3.3 Gram staining solution: see A.2 of Annex A.
3.4 Medium for growing in 7% sodium chloride solution: see A.3 of Annex A.
3.5 Medium and reagent for V-P determination: see A.4 of Annex A.
3.6 Medium and reagent for nitrate reduction: see A.5 of Annex A.
3.7 Medium for D-mannitol fermentation: see A.6 of Annex A.
3.8 Medium for propionate utilization: see A.7 of Annex A.
Foreword i
1 Scope
2 Normative references
3 Diluent, medium and reagent
4 Equipment and glassware
5 Sampling
6 Preparation of specimen
7 Operation procedures
8 Result calculation and report
Annex A (Informative) Medium and reagents
