1 Scope
This standard specifies the enzyme-linked immunosorbent assay (ELISA) method for the determination of aflatoxin B1 in animal feeding stuffs.
This standard is applicable to the determination of aflatoxin B1 in various feed materials, formula feeds and concentrate feeds.
The detection limit of this standard is 0.1μg/kg.
2 Normative Reference
The following standard contains provisions which, through reference in this standard, constitute provisions of this standard. For dated reference, subsequent amendments to (excluding correction to), or revisions of, any of these publications do not apply. However, all parties coming to agreements based on this standard are encouraged to investigate the possibility of applying the most recent editions of the standards. For undated references, the latest edition of the normative document referred to applies.
GB/T 20195 Animal Feeding Stuffs - Preparation of Test Samples (GB/T 20195-2006, ISO 6498:1998, IDT)
3 Principle
Foreword I
1 Scope
2 Normative Reference
3 Principle
4 Reagents and Materials
5 Apparatus and Equipment
6 Analysis Procedure
7 Precision
Appendix A (Informative) Extraction Method for Concentrate Feed
Appendix B (Informative) Specimen Solution Dilution