Food microbiological examination - Sampling and sample processing of grain products
1 Scope
This standard specifies the sampling and sample processing methods of grain products.
This standard is applicable to the sampling and sample inspection of grain products.
2 Equipment and materials
2.1 Sampling tools
The sampling tools shall be made of stainless steel or other materials with appropriate strength, and they shall have smooth surfaces, and rounded corners, but not joints. The sampling tools shall be cleaned and sterilized, while large tools that cannot be sterilized shall be disinfected, and kept dry before use. The sampling tools include stirring appliances, sampling scoops and spoons, knives, sampling drills, scissors, tweezers, etc.
2.2 Sample containers
The material (such as glass, stainless steel, and plastic) and structure of the sample containers shall be able to fully make the samples the original state. The containers and their lids shall be clean, sterile, and dry. The sample containers shall have sufficient volume to allow the samples to be thoroughly mixed prior to inspection. The sample containers also include sampling bags, sampling tubes, sampling bottles, and the like.
2.3 Other supplies
They may include alcohol lamp, thermometer, aluminum foil, sealing film, marker, and sampling registration form.
3 Sampling
3.1 Sampling principles and plans
The sampling principles and plans shall be implemented in accordance with the requirements of GB 4789.1.
The number (n) of the specimens to be sampled shall meet the requirements of relevant food safety standards. The sampling volume for each sample shall not be less than the volume of the sample for 5 × inspection units, or shall be determined according to the inspection purpose. The requirements for the sampling of one food sample are specified as follows:
3.2 Prepackaged grain products
3.2.1 Individually packaged grain products of less than or equal to 1,000g: individually packaged products from the same batch shall be sampled.
3.2.2 Individually packaged grain products of more than 1,000g, the individual package may be adopted, or a suitable amount of sample may be taken from different parts of the same packaging using the aseptic sampling tool and placed into a single aseptic sampling container as one food sample.
3.3 Grain products in bulk or produced on site
The appropriate products shall be sampled by an aseptic sampler from the five parts of a package, and the samples are placed in an aseptic sampler as one food sample.
3.4 Sample storage and transportation
The requirements of GB 4789.1 shall apply.
4 Preparation of samples
4.1 Opening of packages
4.1.1 Products in can or bottle: Check the integrity, tightness and material of the outer package, clean the outer package surface of the bottle or can with flowing water under the condition that there is visible dirt on the surface, then disinfect the lid of the can or bottle and its nearby area with 75% alcohol cotton ball, and then open the can or bottle with a sterilized can opener; the products in can or bottle that are not completely sealed or unsuitable for flowing water cleaning shall be disinfected with 75% alcohol cotton balls on the lid and nearby area of the can or bottle, and then opened with sterile operation.
4.1.2 Products in bag: Use a 75% alcohol cotton ball to wipe and disinfect the opening of the bag or the location where the sample is taken out, as well as the surrounding area. Use sterilized scissors to cut open the bag or remove the zip ring.
Contents Foreword i 1 Scope 2 Equipment and materials 3 Sampling 4 Preparation of samples 5 Inspection
